Increasing cases of antibiotic resistance encourage researchers to find new bioactive compounds.
The source of the newly developed antibiotics is utilizing endophytic bacteria. BA1 isolate is an endophytic
bacterium from Lansano plant (Pterocarpus indicus Willd) which has high antimicrobial activity. Antibiotic
compounds are produced through a fermentation process. The purpose of this study was to optimize the
fermentation conditions of endophytic bacteria Lansano isolate BA1 in producing antibiotic compounds.
Optimization of the fermentation time was carried out for 72 hours. Optimization of starter fermentation using
concentrations (1%, 5%, 10%, and 15%). While the optimization of the fermentation medium used the types of
medium MH, NB, LB, and LBB. Antibiotic activity test (antifungal and antibacterial) was carried out using the
paper disc diffusion method. The results showed that the endophytic bacteria Lansano isolate BA1 had a growth
curve consisting of: a lag phase at 0 to 4 hours of fermentation, a log phase at 8 to 12 hours of fermentation, and
a stationary phase starting after fermentation. 12 o'clock. The best fermentation medium for producing
antibacterial compounds by endophytic bacteria Lansano isolate BA1 is MH medium. There was an effect of the
duration of fermentation of the endophytic bacteria Lansano isolate BA1 on different types of medium to
produce antibiotic compounds. The best concentration of starter fermentation for producing antibiotic
compounds by endophytic bacteria Lansano isolate BA1 was 10% for antifungal and 5% for antibacterial.
Keywords: Optimization, Endophyte Lansano bacteria (Pterocarpus indicus Willd), BA 1 isolate, Antibiotic